Slic-seq for A-to-I edited transcripts enrichment

Adenosine-to-inosine (A-to-I) RNA editing is a post-transcriptional processing event involved in diversifying the transcriptome responsible for various biological processes. In this context, we developed a new biochemical method that enriches the inosine-containing RNA. The objective is the accurate identification of A-to-I editing sites, eliminating false positives caused by RNA-DNA differences. This method was applied to three neurological diseases, demonstrating that A-to-I editing sites significantly decreased in neuronal activity genes. Overall design: 51 samples are analyzed(cell line data is mainly used to verify the reliability of the method, mouse brain tissue and mouse brain tissue is mainly used to study disease models)