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Characterization of AAEL012437 (Veneno)-interacting proteins.

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NIAID Data Ecosystem2026-03-10 收录
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To investigate the repertoire of proteins interacting with the Aedes aegypti TUDOR protein AAEL012437 (Veneno), we transiently expressed a GFP-Veneno transgene in Aag2 cells. These cells were either infected with the alphavirus Sindbis virus (SINV) or mock-infected. Subsequently, cells were lysed and processed for immunoprecipitation using GFP-TRAP beads to purify Veneno-complexes. The same approach was followed for a truncated transgene lacking the first 205 amino acids (C206).

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2018-12-12
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