Human Long Intergenic Noncoding RNA Linc-ADAL Knockdown in Mature Adipocytes using Lentiviral ShRNA

Thousands of human long intergenic noncoding RNAs (lincRNAs) have been detected in human adipose tissue. Here we characterized the function of one human adipse lincRNA, linc-ADAL (chr5:115292235-115296985) in mature adipcoytes through loss-of-function studies. Our results indicate that knockdown of linc-ADAL in differentiated adipocytes modulated expression of lipid metabolism genes. Overall design: Lentiviral based shRNAs were used to knockdown linc-ADAL in differentiated ASC-derived adipocytes. ASC-adipoctes were transduced on day 8 of adipogenic differentiation. Cells were harvested for RNA extraction on day 6 post-transduction.