Hepatic Gene Expression Induced by CREB-H-ΔTC

The cAMP-response element binding protein H (CREB-H) is a transcription factor expressed in liver and intestinal tissues. It is well known for regulating many lipid and glucose metabolism genes. Our group aimed to screen for additional or potential genes that may be downstream of CREB-H transcription. To prevent inaccuracy arisen from endogenous CREB-H expression, we used CREB-H-knockout (CREB-H KO) mice for this study. We intraperitoneally injected liver-specific adeno-associated virus that was conjugated to the CREB-H-ΔTC transcript i.e., the active form of CREB-H (AAV-CREB-H- ΔTC) which regulates gene transcription into CREB-H KO mice. Whereas, for the control group, CREB-H KO mice received GFP expression (AAV-GFP). The sample size was n=3 per group. Mice were sacrificed two weeks after the injection. Liver tissues were extracted and processed for microarray analysis.