(A) SDS-PAGE and Coomassie blue staining of the whole cell lysate corresponding to E. coli BL21 transformed with pCold-TF-AcLAP plasmid (lane 1), soluble fraction of the sonicated cell lysate (lane 2)

NIAID Data Ecosystem40

Purification and characterization of a mammalian myosin I.

蛋白质纯化

酶学表征

Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction

PubMed Central1992-01-15 更新30

Streptomyces albus J1074 TG1-mCherry-17 genome sequencing. Streptomyces albus strain:J1074 TG1-mCherry-17

链霉菌基因工程

异源表达

Genome sequencing of engineered Streptomyces strains to understand actinophage integrase substrate specificity and accuracy and correlation between gene locations and heterologous gene expression leve

NIAID Data Ecosystem60

E-GEOD-59814

酵母代谢工程

异源表达

RNA-seq of ACS (acetyl-CoA synthetase)-deficient yeast strain IMY104 engineered to express and assemble a pyruvate dehydrogenase complex (PDH) from Enterococcus faecalis in its cytosol against an ACS+

NIAID Data Ecosystem30

Purification of heterologously expressed Coh00831 and Coh01133.

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