Supporting data for "Streptococcus pyogenes Cas9 (SpCas9) and Staphylococcus aureus Cas9 (SaCas9) Engineering with Enhanced Genome Targeting Accuracy"

Cas9 engineering were conducted on SpCas9 and KKH-SaCas9 that contributed to two specificity improved variants: Opti-SpCas9 and KKH-SaCas9-SAV1. Experiments including GFP disruption assays and T7E1 assays were conducted for editing activity characterizations. Experimental data of the engineered Cas9 variants are included here.

对SpCas9和KKH-SaCas9进行了Cas9工程改造，从而产生了两种特异性提升的变体：Opti-SpCas9和KKH-SaCas9-SAV1。为表征编辑活性，进行了包括GFP破坏实验和T7E1实验在内的实验。此处包含了改造后的Cas9变体的实验数据。