Whole transcriptome analysis of mouse aortas and kidneys with or without deletion of the EGFR in endothelial cells after 18 weeks of high fat diet

Vascular EGF receptors (EGFR) are supposed to be relevant for function and structure of arterial vessels. Recently, we showed in genetic mouse models that vascular smooth muscle (VSMC) EGFR is required for proper physiological function and structure as well as for pathophysiological alterations induced by angiotensin II or obesity. The importance of endothelial (EC) EGFR in vivo is unknown. Therefore we analyzed the impact of EC-EGFR knockout on vascular and renal function under control condition as well as in obesity in comparison to VSMC-KO. Overall design: RNASequencing of aorta and kidney samples from wild-type mice and mice with knock-out of their endothelial cells-EGFR. Mice were fed with standard or high fat diets (60% fat share of energy versus 10%). Raw RNASequencing data were treated successively with HISAT2 (mapping) and featureCounts (counting).