AT3 RNA-seq

ER+ breast cancer is the most prevalent subtype of the disease with improved prognosis thanks to advances in target therapies against the main driver of proliferation, estrogen receptor (ER). Despite all the advances in the study of this subtype, murine models that allow to study the interaction of tumor cells with the stromal compartment are merely absent. In this study we aimed to ectopically overexpresse the protein ER in AT3 murine cell line and check the ability to resemble ER+ breast cancer cells. We compared the transcriptomic profile of ER-OE cells versus control. Overall design: AT3 cells were transduced with vector control or ER overexpressing construct. Both cell lines were treated with 10 nm of E2 during 24 h. Cell pellets were lysed and RNA extracted for RNA sequencing. Transcriptomic changes upon ER overexpression together with E2 treatment were analyzed.