Effect of two type of microalgae on rumen characteristics, methane production, and microbiome in vitro

Coccomyxa sp. KJ is a unicellular green microalga isolated in Japan. Coccomyxa sp. KJ accumulates many lipids when cultured under nitrogen-deficient conditions, whereas it contains high amounts of nitrogen when cultured under nitrogen-sufficient conditions. The aim of this study was to determine the effects of two types of Coccomyxa sp. KJ on in vitro ruminal fermentation, digestibility, methane production, and the ruminal microbiome.