Transcriptome change mediated by tumor-intrinsic PRC2 inactivation in transplant murine breast cancer AT3 tumor model in C57BL/6J mice

We used CRISPR/Cas9-mediated knockout of PRC2 core components, Eed and generated PRC2-isogenic murine mammary tumor model (AT3, sgCon vs. sgEed ) amenable for syngeneic transplant in C57BL/6J mice. Transcriptome analysis of the explanted PRC2-wt (sgCon) and PRC2-loss (sgEed) AT3 tumors by RNA-seq demonstrated that PRC2 loss led to the upregulation of various developmental pathways, and the downregulation of both innate and adaptive immune response pathways. Overall design: mRNA profiles of AT3 sgCon and sgEed tumors after mammary fat pad grafting in C57BL/6J mice