Mitochondria enriched proteome from heart of heart Sod2 knockout mice LC MS/MS

Superoxide dismutases (SOD) convert superoxide to hydrogen peroxide and therefore provide an important ROS defence mechanism. SOD2 is the sole superoxide dismutase in mitochondrial matrix and thus SOD2 knockout mice can be utilized to analyse what effects increased oxidative stress has on mitochondrial proteome. To this end, we purified heart mitochondria from heart Sod2 knockout mice (Sod2 Ckmm cre) and controls and analysed their proteome with label free LS MS/MS.

超氧化物歧化酶（Superoxide dismutases, SOD）可将超氧阴离子转化为过氧化氢，从而构成重要的活性氧（Reactive Oxygen Species, ROS）防御体系。SOD2是线粒体基质中唯一的超氧化物歧化酶，因此可借助SOD2敲除小鼠探究氧化应激增强对线粒体蛋白质组的影响。为此，我们从心脏特异性Sod2敲除小鼠（Sod2 Ckmm cre）及其对照小鼠中分离纯化心脏线粒体，并采用无标记液相色谱-串联质谱（label-free LS MS/MS）对二者的蛋白质组进行分析。

搜集汇总

背景与挑战

背景概述

该数据集通过无标记LC MS/MS技术，分析了心脏SOD2敲除小鼠心脏线粒体富集蛋白质组，旨在研究氧化应激增加对线粒体蛋白质组的影响。数据集聚焦于小鼠心脏组织，由Xinping Li团队提交，为探究线粒体抗氧化防御机制提供了关键蛋白质组学数据。