Cydrasil-v3-reference-alignment.afa

This is a curated alignment generated using SSU-Align 0.1.1 with default parameters and masked using the ssu-mask feature of SSU-Align with per-alignment calculated masks. The alignment was then manually examined in Geneious version 8 to remove duplicate aligned sequences, non-16S rRNA sequences, and alignment errors. Sequences that were poorly aligned (typically due to the unlabeled inclusion of the ITS and/or 23S regions) were trimmed and removed if their length fell below the 1100 base pair threshold. The alignment was generated using the FASTA file cydrasil-v3-sequence-list.fasta found within the Cydrasil 3.0 figshare project. This alignment file is in aligned FASTA format.