AI-Guided Plasticizer Biodegradation Genomics Dataset of Rhizobium pusense KSSKSLAB04

This repository contains the complete analysis dataset associated with the hybrid whole-genome sequencing and functional characterization of Rhizobium pusense strain KSSKSLAB04, a plasticizer-degrading bacterium.The genome was generated using a hybrid sequencing strategy integrating Oxford Nanopore long-read sequencing and PacBio Onso short-read sequencing, resulting in a high-quality ~5.33 Mb assembly comprising 5,043 protein-coding genes (CDS), 12 rRNA genes, 57 tRNA genes, and 1 tmRNA (total 5,113 annotated genes). This Figshare repository includes:• Final polished genome assembly (FASTA format)• Genome annotation files (GFF3, CDS nucleotide sequences, protein FASTA)• Assembly quality statistics (QUAST, BUSCO, CheckM2 summaries)• KEGG and KofamScan pathway reconstruction outputs• eggNOG functional annotation results• Pan-genome analysis outputs and gene presence/absence matrices• Mobile genetic element analysis (ISEScan, genomic islands, prophage predictions)• antiSMASH secondary metabolite biosynthetic gene cluster predictions• FastANI taxonomic validation results• Variant calling outputs (VCF and annotated SNP tables)• Oxygenase and esterase identification datasets• Machine-learning datasets and analysis outputs for plasticizer hydrolase predictionRaw sequencing reads are available in NCBI SRA under the associated BioProject. The assembled genome is deposited in NCBI GenBank (Accession: JBUXKN000000000). All custom scripts and bioinformatics pipelines are available on GitHub.This dataset is provided to ensure full reproducibility, transparency, and reusability of the genomic, comparative, and machine-learning analyses described in the associated manuscript.