Appendix 1 in Integrative taxonomy reveals four new species in the superdiverse genus Macrobiotus (Tardigrada: Eutardigrada: Macrobiotidae)

Appendix 1 (continued on next five pages). Sequences used for phylogenetic analyses. Sequences obtained in this study are indicated in bold, and asterisks denotes Macrobiotus C.A.S. Schultze, 1834 sequences that was misidentified by previous authors, as noted by (Stec 2024). Shaded rows at the end of the table indicate outgroup taxa. Species18S28SITS-2COISourceMacrobiotus witalinskii MG. 001.01PX789912PX789920PX789928PX794742This studyMacrobiotus witalinskii MG. 001.02PX789913PX789921PX789929PX794743This studyMacrobiotus hoianicus VN. 004.01PX789914PX789922PX789930PX794744This studyMacrobiotus hoianicus VN. 004.02PX789915PX789923PX789931PX794745This studyMacrobiotus surmaczi VN. 003.01PX789916PX789925PX789932PX794746This studyMacrobiotus surmaczi VN. 003.02PX789917PX789924PX789933PX794747This studyMacrobiotus dalaticus VN. 002.01PX789918PX789927PX789934PX794748This studyMacrobiotus dalaticus VN. 002.02PX789919PX789926PX789935PX794749This studyMacrobiotus vattenrikensePX093663PX093644PX093655Hulterström et al. 2025Macrobiotus anshuiOQ525805OQ526003OQ525803Kayastha et al. 2025Macrobiotus sharapoviPV283176PV283177PV283179PV282532Polishchuk et al. 2025Macrobiotus olgae 1PQ461834PQ276069Rocha et al. 2025Macrobiotus olgae 2PQ461835PQ276070Rocha et al. 2025Macrobiotus kathyae 1PP391285PP391287PP391289PP386934Massa & Vecchi 2024Macrobiotus kathyae 2PP391286PP391288PP391290PP386935Massa & Vecchi 2024Macrobiotus ovovittatus GL. 001.01OR543310OR543318OR543314OR544395Stec 2024Macrobiotus ovovittatus GL. 001.02OR543311OR543319OR543315OR544396Stec 2024Macrobiotus mileri IL. 001.01OR543312OR543320OR543316OR544397Stec 2024Macrobiotus mileri IL. 001.02OR543313OR543321OR543317OR544398Stec 2024Macrobiotus mileri IL. 001.03OR544399Stec 2024Macrobiotus mileri IL. 001.04OR544400Stec 2024Macrobiotus kosmali M8.1OP142472OP143765OP153786OP141639Kayastha et al. 2023Macrobiotus kosmali M8.2OP142473OP143766OP141640Kayastha et al. 2023Macrobiotus hupingensis *MW183923MZ470349MZ474842MW186952Yuan et al. 2022Macrobiotus dolosus C3209_2OP596290OP561772Bertolani et al. 2023Macrobiotus dolosus C3209_US2OP561773Bertolani et al. 2023Macrobiotus dolosus C3209_4OP561774Bertolani et al. 2023Macrobiotus dolosus C3581_V6OP596292OP561775Bertolani et al. 2023Macrobiotus dolosus C3209_1OP596289Bertolani et al. 2023Macrobiotus dolosus C3581_V5OP596291Bertolani et al. 2023Macrobiotus sidereus C2796_2OP596293OP561776Bertolani et al. 2023Macrobiotus sidereus C3282_UFKOP561777Bertolani et al. 2023Macrobiotus sidereus C3282_ UFLOP561778Bertolani et al. 2023Macrobiotus sidereus C3282_ UFMOP561779Bertolani et al. 2023Macrobiotus sidereus C3282_ UFNOP596294OP561780Bertolani et al. 2023Macrobiotus sidereus C3282_ UFOOP561781Bertolani et al. 2023Macrobiotus sidereus C3282_ UFPOP561782Bertolani et al. 2023Macrobiotus fonturai C2861_5OP596295OP561783Bertolani et al. 2023Macrobiotus fonturai C2861_ US1OP596296OP561784Bertolani et al. 2023Macrobiotus muralis C2861_FGOP596297Bertolani et al. 2023Macrobiotus muralis C2861_6OP596298Bertolani et al. 2023Macrobiotus cf. muralis C3251_2OP596299OP561785Bertolani et al. 2023Macrobiotus cf. muralis C3251_3OP596300OP561786Bertolani et al. 2023Macrobiotus cf. muralis C3251_FAOP596301OP561787Bertolani et al. 2023Macrobiotus cf. muralis C3251_4OP561788Bertolani et al. 2023Macrobiotus cf. muralis C3251_5OP561789Bertolani et al. 2023Macrobiotus hufelandi C2953_ A02OP596302HQ876586Bertolani et al. 2011, 2023Macrobiotus cf. hufelandi C2959_A01OP596303HQ876590Bertolani et al. 2011, 2023Macrobiotus cf. hufelandi C2959_A02OP596304HQ876591Bertolani et al. 2011, 2023Macrobiotus vladimiri C2688_ A02OP596305HM136932Bertolani et al. 2011, 2023Macrobiotus terminalis C2868_ N02OP596308JN673959Bertolani et al. 2011, 2023Macrobiotus cf. nelsonae 1HQ604965Bertolani et al. 2014Macrobiotus cf. nelsonae 2HQ604966Bertolani et al. 2014Macrobiotus rebecchii 1OP479887OP477442Stec 2022aMacrobiotus rebecchii 2OP479888OP477443Stec 2022aMacrobiotus kyoukenus v3ON818312ON818300ON809461Cesari et al. 2022Macrobiotus kyoukenus us3ON818314ON818301ON809462Cesari et al. 2022Macrobiotus kyoukenus us4ON818315ON818302ON809463Cesari et al. 2022Macrobiotus kyoukenus us5ON818316ON818303ON809464Cesari et al. 2022Macrobiotus scoticus DK. 056.01OK663218OK663207OK662989OK663228Vecchi et al. 2022aMacrobiotus scoticus DK. 056.02OK663217OK663206OK662988OK663229Vecchi et al. 2022aMacrobiotus naginae S226_1OK663219OK663230OK663209OK662990Vecchi et al. 2022aMacrobiotus naginae S226_2OK663220OK663231OK663208OK662991Vecchi et al. 2022aMacrobiotus naginae S605_1OK663221OK663232OK663210OK662992Vecchi et al. 2022aMacrobiotus naginae S605_2OK663222OK663233OK663211OK662993Vecchi et al. 2022aMacrobiotus sandrae S859_1OK663223OK663234OK663212OK662994Vecchi et al. 2022aMacrobiotus cf. sapiensOK663226OK663237OK663215OK662997Vecchi et al. 2022aMacrobiotus sandrae 1MW695445HQ876573Bertolani et al. 2011; Marnissi et al. 2021Macrobiotus sandrae 2MW695446HQ876577Bertolani et al. 2011; Marnissi et al. 2021Macrobiotus azzunae 1MW695447MW695454MW698697Marnissi et al. 2021Macrobiotus azzunae 2MW695448MW695455MW698698Marnissi et al. 2021Macrobiotus birendraiMW680641MW680644MW680418MW656266Kayastha et al. 2021Macrobiotus a. groenlandicus 1MZ463664MZ463679MZ463654MZ461006Stec et al. 2022Macrobiotus a. groenlandicus 2MZ463663MZ463678MZ463655MZ461007Stec et al. 2022Macrobiotus a. groenlandicus 3MZ463662MZ463677MZ463653MZ461005Stec et al. 2022Macrobiotus kirghizicus 1MZ463666MZ463672MZ463659MZ461002Stec et al. 2022Macrobiotus kirghizicus 2MZ463665MZ463671MZ463660MZ461003Stec et al. 2022Macrobiotus kirghizicus 3MZ463667MZ463673MZ463661MZ461004Stec et al. 2022Macrobiotus a. ariekammensis 1MZ463668MZ463674MZ463656MZ460999Stec et al. 2022Macrobiotus a. ariekammensis 2MZ463669MZ463675MZ463657MZ461000Stec et al. 2022Macrobiotus a. ariekammensis 3MZ463670MZ463676MZ463658MZ461001Stec et al. 2022Macrobiotus aff. pseudohufelandi PLMN888373MN888358MN888345MN888325Stec et al. 2021aMacrobiotus aff. pseudohufelandi ZAMN888374MN888359MN888345MN888326Stec et al. 2021aMacrobiotus annewintersae 1MW588024MW588030MW588019MW593927Vecchi & Stec 2021Macrobiotus annewintersae 2MW588025MW588031MW588018MW593928Vecchi & Stec 2021Macrobiotus basiatusMT498094MT488397MT505165MT502116Nelson et al. 2020Macrobiotus caelestisMK737073MK737071MK737072MK737922Coughlan et al. 2019Macrobiotus canaricus 1MH063925MH063934MH063928MH057765Stec et al. 2018dMacrobiotus canaricus 2MH063929MH057766Stec et al. 2018dMacrobiotus cf. polonicus 1MW588026MW588032MW588021MW593929Vecchi & Stec 2021Macrobiotus cf. polonicus 2MW588027MW588033MW588020MW593930Vecchi & Stec 2021Macrobiotus cf. recens 1MH063927MH063936MH063932MH057768Stec et al. 2018dMacrobiotus cf. recens 2MH063933MH057769Stec et al. 2018dMacrobiotus crustulusMT261912MT261903MT261907MT260371Stec et al. 2020dMacrobiotus engbergi 1MN443039MN443034MN443036MN444824Stec et al. 2020cMacrobiotus engbergi 2MN443037MN444825Stec et al. 2020cMacrobiotus engbergi 3MN444826Stec et al. 2020cMacrobiotus glebkaiMW247177MW247176MW247180MW246134Kiosya et al. 2021Macrobiotus hannaeMH063922MH063924MH063923MH057764Nowak & Stec 2018Macrobiotus kamilae 1MK737070MK737064MK737067MK737920Coughlan & Stec 2019Macrobiotus kamilae 2MK737921Coughlan & Stec 2019Macrobiotus kristenseniKC193577KC193573Guidetti et al. 2013Macrobiotus macrocalixMH063926MH063935MH063931MH057767Stec et al. 2018dMacrobiotus noongaris 1MK737069MK737063MK737065MK737919Coughlan & Stec 2019Macrobiotus noongaris 2MK737066Coughlan & Stec 2019Macrobiotus papeiMH063881MH063880MH063921MH057763Stec et al. 2018aMacrobiotus paulinaeKT935502KT935501KT935500KT951668Stec et al. 2015Macrobiotus polonicus AT 1MN888369MN888355MN888337MN888317Stec et al. 2021aMacrobiotus polonicus AT 2MN888338MN888318Stec et al. 2021aMacrobiotus polonicus AT 3MN888319Stec et al. 2021aMacrobiotus polonicus SK 1MN888370MN888356MN888332MN888320Stec et al. 2021aMacrobiotus polonicus SK 2MN888333MN888321Stec et al. 2021aMacrobiotus polonicus SK 3MN888334Stec et al. 2021aMacrobiotus porifini 1MT241900MT241897MT246659Kuzdrowska et al. 2021Macrobiotus porifini 2MT241898MT246660Kuzdrowska et al. 2021Macrobiotus porifini 3MT241899MT246661Kuzdrowska et al. 2021Macrobiotus polypiformis 1KX810008KX810009KX810010KX810011Roszkowska et al. 2017Macrobiotus polypiformis 2KX810012Roszkowska et al. 2017Macrobiotus pseudohufelandi 1HQ604989AY598776Bertolani et al. 2014Macrobiotus pseudohufelandi 2HQ604990AY598777Bertolani et al. 2014Macrobiotus rybaki 1MW588029MW588034MW588022MW593931Vecchi & Stec 2021Macrobiotus rybaki 2MW588028MW588035MW588023MW593932Vecchi & Stec 2021Macrobiotus scoticusKY797265KY797266KY797268KY797267Stec et al. 2017bMacrobiotus shonaicus 1MG757132MG757133MG757134MG757136Stec et al. 2018bMacrobiotus shonaicus 2MG757135MG757137Stec et al. 2018bMacrobiotus sottileiMW247178MW247175MW247179MW246133Kiosya et al. 2021Macrobiotus vladimiri FIMN888375MN888360MN888347MN888327Stec et al. 2021aMacrobiotus wandaeMN435112MN435116MN435120MN482684Kayastha et al. 2020Macrobiotus pallarii 1MT809069MT809081MT809094MT807924Stec et al. 2021bMacrobiotus pallarii 2MT809070MT809082MT809095MT807925Stec et al. 2021bMacrobiotus pallarii 3MT809071MT809083MT809096MT807926Stec et al. 2021bMacrobiotus pseudopallarii 1MT809067MT809079MT809091MT807921Stec et al. 2021bMacrobiotus pseudopallarii 2MT809068MT809080MT809092MT807922Stec et al. 2021bMacrobiotus ripperi FIMT809076MT809089MT809103MT807930Stec et al. 2021bMacrobiotus ripperi PLMT809074MT809086MT809100MT807933Stec et al. 2021bMacrobiotus margoae USMT809072MT809084MT809098MT807927Stec et al. 2021bMacrobiotus gretae 1MW588434MW588431MW581665Massa et al. 2021Macrobiotus gretae 2MW588437MW588433MW581668Massa et al. 2021Macrobiotus gretae 3MW588435MW588432MW581666Massa et al. 2021Macrobiotus gretae 4MW588436MW581667Massa et al. 2021Sisubiotus hakaiensis 1OM523054OM523059OM523057OM523181Vecchi et al. 2022bSisubiotus hakaiensis 2OM523055OM523060OM523058OM523182Vecchi et al. 2022bSisubiotus spectabilis FI 1MN888371MN888357MN888331MN888322Stec et al. 2021aSisubiotus spectabilis FI 2MN888323Stec et al. 2021aSisubiotus spectabilis NOMN888372MN888364MN888344MN888324Ste et al. 2021aSisubiotus splendidusPV744336PV744341PV746802Atherton 2025Mesobiotus efa 1OR804457OR805135OR805169OR803035Tumanov et al. 2024Mesobiotus efa 2OR804458OR805136OR805170OR803036Tumanov et al. 2024Mesobiotus efa 3OR804459OR805137OR805171OR803037Tumanov et al. 2024Mesobiotus vulpinus 1OR804461OR805140OR805172OR803040Tumanov et al. 2024Mesobiotus vulpinus 2OR804462OR805141OR805173OR803041Tumanov et al. 2024Mesobiotus occultatus DT 97OR794157OR794158OR803042OR805249Tumanov et al. 2024Mesobiotus aradasiOQ933000OQ932999OQ940183OQ928739Vecchi et al. 2024Mesobiotus huecoensis S2027.1OQ756249OQ756246Vecchi et al. 2023Mesobiotus huecoensis S2027.4OQ756248OQ756247Vecchi et al. 2023Mesobiotus mandalori 1OP829143OP829056OP829058OP825090Erdmann et al. 2024Mesobiotus mandalori 2OP829144OP829057OP825091Erdmann et al. 2024Mesobiotus anastasiaeMT903468MT903612MT903470MT904513Tumanov 2020Mesobiotus hilariaeKT226070KT226108Vecchi et al. 2016Mesobiotus cf. barabanoviMN310392MN310388MN310390MN313170Kaczmarek et al. 2020Mesobiotus datanlanicusMK584659MK584658MK584657MK578905Stec 2019Mesobiotus dilimanensisMN257048MN257049MN257050MN257047Itang et al. 2020Mesobiotus ethiopicusMF678793MF678792MN122776MF678794Stec & Kristensen 2017Mesobiotus fiedleriMH681585MH681693MH681724MH676056Kaczmarek et al. 2020Mesobiotus harmsworthiMH197146MH197264MH197154MH195150Kaczmarek et al. 2018bMesobiotus insanisMF441488MF441489MF441490MF441491Mapalo et al. 2017Mesobiotus occultatusMH197147MH197155MH195152Kaczmarek et al. 2018bMesobiotus philippinicusKX129793KX129794KX129795KX129796Mapalo et al. 2016Mesobiotus radiatus 1MH197153MH197152MH197267MH195147Stec et al. 2018eMesobiotus radiatus 2MH197268MH195148Stec et al. 2018eMesobiotus romaniMH197158MH197151MH197150MH195149Roszkowska et al. 2018Mesobiotus imperialis 1OL257854OL257866OL311514Stec 2021Mesobiotus imperialis 2OL257855OL257867OL311515Stec 2021Mesobiotus marmoreus 1OL257856OL257868OL257861OL311516Stec 2021Mesobiotus marmoreus 2OL257857OL257869OL257862OL311517Stec 2021Mesobiotus marmoreus 3OL257858OL257870OL257863OL311518Stec 2021Mesobiotus skorackiiMW680636MW656257Kayastha et al. 2021Mesobiotus sp. Macro07_042MW751942MW727957Short et al. 2022Mesobiotus cf. furciger Macro 06_296MW751936MW727958Short et al. 2022Mesobiotus cf. furciger Macro 06_310MW751937MW727961Short et al. 2022Mesobiotus cf. furciger Macro 06_313MW751939MW727960Short et al. 2022Mesobiotus cf. furciger CC _MF_4MW751949MW727933Short et al. 2022Mesobiotus cf. furciger ABDC _MF_3MW751944MW727932Short et al. 2022Mesobiotus cf. furciger KPRI _MF_1MW751962MW727934Short et al. 2022Mesobiotus cf. furciger HMI _MF_1MW751957MW727941Short et al. 2022Mesobiotus cf. furciger EBNI _MF_2MW751952MW727937Short et al. 2022Mesobiotus cf. furciger EBNI _MF_4MW751954MW727938Short et al. 2022Mesobiotus cf. furciger PSAI _MF_2MW751967MW727939Short et al. 2022Mesobiotus cf. furciger Macro 06_162MW751934MW727955Short et al. 2022Mesobiotus cf. furciger Macro 06_171MW751935MW727956Short et al. 2022Mesobiotus cf. furciger JN 07_MF_1MW751959MW727951Short et al. 2022Mesobiotus cf. furciger JN 07_MF_4MW751960MW727953Short et al. 2022Mesobiotus cf. furciger JN 07_MF_8MW751961MW727947Short et al. 2022Mesobiotus cf. furciger FN 01_MF_6MW751955MW727945Short et al. 2022Mesobiotus diegoi 1OP142527OP142520OP142514OP143858Stec 2022bMesobiotus diegoi 2OP142526OP142521OP142515OP143857Stec 2022bMesobiotus maklowiczi 1OP142525OP142518OP143855Stec 2022bMesobiotus maklowiczi 2OP142524OP142519OP143856Stec 2022bMesobiotus peterseni 1OP142528OP142522OP142516OP143859Stec 2022bMesobiotus peterseni 2OP142529OP142523OP142517OP143860Stec 2022bMinibiotus ioculatorMT023998MT024041MT024000MT023412Stec et al. 2020bParamacrobiotus areolatusMH664931MH664948MH666080MH675998Stec et al. 2020bTenuibiotus zandraeMN443040MN443035MN443038MN444827Stec et al. 2020c