RNA seq analysis of kupffer cells isolated from mouse fed a high fat diet, deficient for Lpcat3 or not in macrophages

To identify potential role of macrophage LPCAT3 in progression of hepatic steatosis in mice, we isolated Kupffer cells from mice deficient for LPCAT3 in macrophages and fed a high fat diet for 16 weeks Overall design: After 16 weeks of high fat diet, livers were digested by a two-steps perfusion using collagenase and homogenate were centrifugated to eliminate cell pelets containing primary hepatocytes. F4/80 positive cells contained in supernatant were isolated by MACS strategy using anti-F4/80 microbeads. Total RNA was extracted using RNeasy Mini Kit (Qiagen) and immediatly frozen at -80°C.