Data for the article LOM-24-05-0051 published in Limnology and Oceanography Methods by Mellilti et al

Reference : LOM-24-05-0051 Title : Quantifying invasion dynamics: qPCR vs. ddPCR in monitoring the alien invasive bloody-red shrimp Hemimysis anomala and its interaction with perch in Lake Geneva Authors : Sirine Melliti, Marine Vautier, Cécile Chardon, Stéphan Jacquet Correspondence : Stéphan Jacquet, UMR CARRTEL, 75bis Avenue de Corzent, 74203 Thonon-les-Bains Cedex, France. E-mail: [e-mail] This file is composed by 3 different sheets "DNA concentration" sheet: - Calculations to get, from copies/μL reaction, copies/Litre of filtered water for Hemimysis anomala and Perca fluviatilis for ddPCR - Concentrations in copies/L of filtered water for Hemimysis anomala for qPCR "qPCR vs ddPCR" sheet: Log Hemimysis anomala DNA concentrations (copies/L filtered water) for qPCR and ddPCR "LOD-LOQ" sheet: Calculations of the Limits of Detection and Quantitation for the ddPCR based on a range of 8 successive concentrations of Hemimysis anomala with 5 replicates - LOD (the lowest concentration at which the target DNA was detected in at least one replicate) - LOQ (the lowest concentration with a coefficient of variation value of less than 35%)