Jennifer Bestman, Holly Cline (2011) CIL:37034, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #1 (day 1) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged daily over 3 days and shows the development of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本数据集展示了来自白化非洲爪蟾（Xenopus laevis）中枢神经系统视顶盖中发育中的神经元图像 #1（第1天），处于第47发育阶段。该神经元每日进行成像，持续3天，展现了树突和轴突随时间推移的发育过程。在获取图像前约24小时，通过体内电穿孔法将编码绿色荧光蛋白（eGFP）的质粒DNA转染至细胞中。在获取图像时，活体蝌蚪经过麻醉处理，置于载玻片下，通过定制构建的双光子显微镜直接穿过其透明皮肤进行成像。