Genes differentially essential in the M. tuberculosis ΔotsA mutant in absence of trehalose supplementation.

A saturated transposon mutant pool established in the M. tuberculosis ΔotsA mutant background was cultured either in the absence or presence of trehalose, subjected to transposon insertion sequencing (Tn-seq) and analyzed using a non-gene centric method. Genome areas harboring significantly less transposon insertions in absence of trehalose are shown. One genome area comprising most of gene Rv1845c harbored significantly more transposon insertions in absence of trehalose (i.e. appearing essential only in presence of trehalose) and is highlighted in grey.