Additional file 4 of A real-time quantitative polymerase chain reaction for the specific detection of Hammondia hammondi and its differentiation from Toxoplasma gondii

Additional file 4: Table S4. Summary of the testing of forward and reverse primers and appropriate probes to establish a H. hammondi real-time PCR. Two SybrGreen assays per primer pair revealed mean ΔCt values including SD and coefficient of variation (CV) for a borderline H. hammondi-positive DNA control and water as a negative control (mean ΔCtpos-neg; SD ΔCtpos-neg; CV of ΔCtpos-neg). For optimal primers (ΔCtpos-neg values of > 4 and a CV 1000 were further followed and assessed for the mean Ct value of the borderline H. hammondi-positive DNA control. The final primer probe combination used in Hham-qPCR1 consisted of the primers Hham275F and Hham81R and the probe Hham222P (Fig. 2; Additional file 3: Table S3).