Type 4 resistant potato starch alters the microbiome, colon and cecal gene expression, and resistance to Citrobacter rodentium infection in mice fed a Total Western Diet

Mice were fed a Total Western Diet based on the National Health and Nutrition Examination Survey (NHANES) 50th percentile intake levels for Americans supplemented with 0, 2, 5 or 10% type 4 resistant potato starch before being infected with Citrobacter rodentium to induce bacterial colitis. Changes in the cecal microbiome were investigated using 16S sequencing, and changes in cecum and distal colon gene expression were evaluated using RNASeq analysis.