PCR amplification and microarray detection of orthobunyaviruses.

Viral RNA derived from cell culture or infected mosquitoes were PCR amplified using GSPs. Amplicons were analyzed using the ArboChip5.1 microarray. POS, positive PCR amplification; NEG, negative PCR amplification; yes, detected by microarray; no, not detected by microarray; n.t., not tested.aViruses propagated in cell culture and identified by microarray to species unless otherwise noted.bVirus-infected mosquito used for microarray evaluations, microarray detected at least one target for each virus.cArboChip5.1 does not include probes specific to the target.dGSP set not specific for this clade.eCEV clade detection only.