Trophic niche drives the evolution of craniofacial shape in Trinidadian guppies

Diverse clades of fishes adapted to feeding on the benthos repeatedly converge on steep craniofacial profiles and shorter, wider heads. But in an incipient radiation, to what extent is this morphological evolution measurable and can we distinguish the relative genetic vs. plastic effects? We use the Trinidadian guppy (Poecilia reticulata) to test the repeatability of adaptation and the alignment of genetic and environmental effects shaping poecilid craniofacial morphology. We compare wild-caught and common garden lab-reared fish to quantify the genetic and plastic components of craniofacial morphology across four populations from two river drainage systems (n=56 total). We first use microCT to capture 3D morphology, then place both landmarks and semilandmarks to perform size-corrected 3D morphometrics and quantify shape space. We find a measurable, significant, and repeatable divergence in craniofacial shape between high predation invertivore and low predation detritivore populations. A..., Collection and animal husbandry
We collected female and male guppies, Poecilia reticulata, from four distinct populations in the Northern Range mountains on the island of Trinidad in spring 2013 and spring 2014. Guppies were collected from two independent drainages: Aripo river (southern slope) and Yarra river (northern slope) (Figure 1A); these watersheds are completely isolated from one another, with no admixture occurring over the mountain divide (but some can occur within each drainage).
The specific populations collected were from benthic detritivore, or low predation, and invertivore, or high predation, sites within the Aripo and Yarra drainages (four populations total). Twenty females and five males were collected at each of the four sites (n = 100 total); the fish were transported in bottles filled halfway with fresh stream water and treated with 2mg Tetracycline (1mg/L) and StressCoat (according to manufacturer directions) to minimize infection during transportation. The fish w..., , # Data from: Trophic niche drives the evolution of craniofacial shape in Trinidadian guppies

<https://doi.org/10.5061/dryad.95x69p8rw>

This is the morphometric data set that was used to attain the results presented in \"Trophic niche drives the evolution of craniofacial shape in Trinidadian guppies\". There are 4 main components to the data set: 1) 3D models of guppy skulls (.ply format), 2) landmark files that correspond to each 3D model (.fcsv output from 3D Slicer), 3) an R code that runs the analysis on this data, and 4) supporting files used by the code. Given these data and the R file, any user can output the statistical and graphical results presented in the manuscript.

## Description of the data and file structure

3D files' filename structure:

Each 3D file is named to reflect the ecological context of that individual. Specifically, files have several elements that are separated by underscore characters. These are:

* Aripo or Yarra- Reflects the river drainage that the fish...,