Enteroendocrine cell lineages that differentially control feeding and gut motility

Enteroendocrine cells (EECs) are specialized sensory cells of the gut-brain axis that are sparsely distributed along the intestinal epithelium. Intestinal EECs of Ngn3-Cre;tdTom and NeuroD1-Cre;tdTom mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of tdTomato signal and analyzed using scRNAseq.肠内分泌细胞（Enteroendocrine cells, EECs）是肠-脑轴的特化感觉细胞，沿肠上皮呈稀疏分布。研究人员通过荧光激活细胞分选术（Fluorescence-activated cell sorting, FACS），依据tdTomato荧光信号的有无，分离获取Ngn3-Cre;tdTom与NeuroD1-Cre;tdTom小鼠的肠内分泌细胞，并采用单细胞RNA测序（single-cell RNA sequencing, scRNAseq）完成相关分析。