Single-cell RNA-sequencing of human intestinal organoid cells

We focused on a rare cell population of human intestine, the BEST4+ cells. Using human intestinal organoid as a model, the BEST4+ cells could be differentiated, FACS-enriched and analyzed by scRNA-seq, together with the BEST4- cell lineages such as the enterocytes, goble cells and EECs. Human intestinal organoids were cultured in differentiation medium with/without IFN-gamma. BEST4+/- cells derived from the differentiated orgnaoids were FACS sorted and individally collected in the 384 well plates for SORT-seq based scRNA-seq analysis.