Immunoglobulin secretion influences the composition of chicken caecal microbiota

In this study we tested interactions between chicken immunoglobulins and caecal microbiota in two different experiments. First we determined which chicken caecal microbiota was coated by immunoglobulins and next, using Ig deficient chickens, we identified taxa, colonisation of which was dependent on immunoglobulin expression.Using cell sorting followed by sequencing of V3/V4 variable region of 16S rRNA, bacterial species with increased or decreased immunoglobulin coating were determined. Next we determined caecal microbiota composition in immunoglobulin knockout chickens. We found out that immunoglobulin coating was common and major taxa were coated with immunoglobulin.