List of the primers used for obtaining DNA templates for T7 co-transcription/cleavage reactions.
收藏Figshare2015-12-03 更新2026-04-29 收录
下载链接：
https://figshare.com/articles/dataset/Dead_Element_Replicating_Degenerate_R2_Element_Replication_and_rDNA_Genomic_Turnover_in_the_Bacillus_rossius_Stick_Insect_Insecta_Phasmida_/1351721
下载链接
链接失效反馈官方服务：
资源简介：
Primers have been utilized to generate: i) the original templates (OT), ii) the “G” to “A” mutation in the functional element ribozyme (G>Afun), iii) the “A” to “G” mutation in the degenerate element ribozyme (A>Gdeg), iv) the partial J1/2 loop deletion in the degenerate element ribozyme (J1/2 loop del), v) the partial J1/2 loop deletion plus “A to G” double mutation (J1/2 loop del+A>Gdeg). For J1/2 loop del and J1/2 loop del+A>Gdeg final products, a nested PCR was performed involving first the BrLoopDel+BrDeg_227REV pair, then T7/28S(G to A)+BrDeg_170REV and T7/28S(A to G)+BrDeg_170REV, respectively. (DOCX)
创建时间：
2015-12-03
