Subcortical modulation of hippocampal activity, single cell recordings in head-restrained, behaving mice

Cortical circuits display different functional states under the influence of subcortical modulatory inputs. In our study we aimed to uncover fundamental aspects of this subcortical modulation by focusing on the effect of vGlut3 positive median raphe neurons on the hippocampal circuitry. Single neuron activity as well as population patterns were recorded the dorsal CA1 region in head-restrained, behaving mice, while vGlut3 positive raphe-hippocampal terminals or their source neurons in the median raphe were stimulated. This dataset complements our larger database of hippocampal neurons recorded and filled in hippocampal slices, mainly by characterizing the spontaneous and evoked physiological behavior of the cells under in vivo conditions.