noRNAlize: SHAPE data normalization software

This project is a data analysis package to analyze and normalize SHAPE data. Traditionally, SHAPE requires the addition of a 3' hairpin to the RNA for normalization. noRNAlize elminates the need for this experimental step by performing a global analysis of the SHAPE data, and establishing mean protection values. This is particularly important when SHAPE analysis is used to map crystal contacts in crystal structures as illustrated here. <br/><br/>This project includes the following software/data packages: <br/> <ul> <li> <a href="https://simtk.org/frs?group_id=159#pack_191">v1.0 </a> : Alpha Release or noRNAlize </li> </ul>

本项研究旨在构建一套数据分析软件包，用于分析并标准化 SHAPE 数据。传统上，SHAPE 分析需对 RNA 分子添加 3' 环状发夹结构以进行标准化处理。noRNAlize 通过对 SHAPE 数据进行全局分析并建立平均保护值，消除了此实验步骤的需求。这在 SHAPE 分析被用于绘制晶体结构中的晶体接触点时尤为重要，如本例所示。本项目包含以下软件/数据包：

<ul>
<li><a href="https://simtk.org/frs?group_id=159#pack_191">v1.0</a> : Alpha 版本或 noRNAlize</li>
</ul>