RNA Polymerase IV sensitizes the chromatin via histone modifications modulating protein coding genes [Degradome]

Knock down of RNA polymerase IV in rice caused pleiotropic reproductive defects. We investigated the molecular effects of loss of pol IV in different rice tissues by profiling sRNA and mRNA transcriptomes, DNA methylomes, histone modifications and degradome sequencing. These analyses revealed the multifaceted roles of RNA polymerase IV in bringing about regulation of protein coding genes. Overall design: We have performed degradome sequencing in the pre-emerged panicle of Wt and pol IV kd plants to profile the differences in the post transcriptional silencing mechanism of RNA silencing upon loss of pol IV.