Data from: Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins

# Data from: Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins [https://doi.org/10.5061/dryad.s4mw6m9cj](https://doi.org/10.5061/dryad.s4mw6m9cj) The data made available in this data set support the conclusions made in the publication in Stem Cell Research & Therapy entitled "Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins". ## Description of the data and file structure The data provided in this dataset support the conclusions of each figure in the publication. Hence, the dataset is structured according to the figure numbers of the publication. Zip files are entitled according to the figure number of the publication (Figure 2 Data, Figure 3 Data, etc.). Files are named to be self-explanatory. Zip files related to figures that include graphs in the publications (Figure 5, Figure 7 and Figure 8) contain Excel files showing the raw data and GraphPad Prism files demonstrating the graphs used in the figure. The Zip file corresponding to Figure 4 contains a FlowJo workbook with the flow cytometry data, as well as the raw .fcs files. Flow data is structured into 2 groups (Fibroblast-iPSC and PBMC-iPSC) with 3 subgroups each (EB, MB and undifferentiated iPSCs as neg. control). Each subgroup has 14 tubes. Tube 1 is negative control with no fluorochromes, tubes 2-7 are isotype controls, tubes 8-13 are fluorescence minus one controls (FMO) and cells tube 14 are stained with the full antibody panel. Fibroblast-derived iPSC are sometimes referred to as "GM28404” or “Fibroblast-iPSC”, PBMC-derived iPSC are sometimes referred to "MCND-S2" or “PBMC-iPSC” within the dataset. Additionally, the embryoid-body based differentiatoin method (EB) is sometimes referred to as "Rössler" while the monolayer-based method (MB) is sometimes referred to as "STEMdiff". ## Code/Software As mentioned above, flow cytometry data is stored in a FlowJo workbook file, raw quantitative data is provided in Excel sheets, graphs used in figures are stored in GraphPad Prism files.