An integrated approach to understanding noise stress in two auditorily diverse species of freshwater fish

Anthropogenic noise can have negative consequences on a fish’s ability to survive and reproduce and has been increasing over the past decades. To determine the integrated effects of anthropogenic noise on two auditorily diverse freshwater fish species, yellow perch (Perca flavescens) and spottail shiner (Notropis hudsonius), the current study assessed behavioural, physiological, and transcriptional metrics. Fish were exposed to anthropogenic noise or control sounds in a semi-captive field setting, and behavioural changes, blood cortisol levels, and relative transcription were analyzed for effects of anthropogenic noise. Results showed little effect of noise on yellow perch; however, spottail shiners were affected by noise at multiple levels of organization. Additionally, blood cortisol levels showed indications of handling stress in both species, overriding any potential effects of noise. For the first time, the current study differentiates stress effects from handling and those induced..., , , # An integrated approach to understanding noise stress in two auditorily diverse species of freshwater fish

[https://doi.org/10.5061/dryad.k6djh9wgv](https://doi.org/10.5061/dryad.k6djh9wgv)

## Description of the data and file structure

This dataset includes raw data files for transcriptional, physiological, and behavioural data for a study conducted on yellow perch and spot-tail shiner that were exposed to acute noise. Each file contains a “dictionary tab” defining variables found in the sheet.

### Files and variables

#### File: BehaviourDataNoFormatting.xlsx

**Description:** Swimming and startle data for yellow perch and spot tail shiner exposed to noise. 

##### Variables

* Variables are defined within the \"dictionary\" tab on the excel sheet

#### File: PerchShinerCortisolData.xlsx

**Description:** Data sheet for blood cortisol data given in (ng/ul)

##### Variables

* Variables are defined within the \"dictionary\" tab on the excel sheet

#### File: PerchRelativeTranscription...,