Dynamic change of ac4C in mouse hippocampus during learning and memory

N4-acetylcytidine (ac4C), the only known form of RNA acetylation, has recently been identified as an epitranscriptomic mechanism regulating mRNA stability and translation efficiency. However, the function and regulation of ac4C in the brain remain largely unknown. Here we performed transcriptome-wide mapping of ac4C in the hippocampus of adult mice that were trained in the Morris water maze, a protocol for learning and memory. We identified ~2100 ac4C-modified mRNAs that were upregulated after memories were formed but returned to normal levels after memories were forgotten. N4-acetylcytidine (ac4C) for acetylation modifiction of mRNA in hippocampus tissues (homogenates and synapse)