Aged polyethylene microplastics modulate herbicide and antibiotic bioavailability and plant responses: a case study with glyphosate and tetracycline

The dataset contains raw and processed results obtained during hydroponic exposure experiments with Brassica napus L., conducted to assess the effects of aged polyethylene microplastics (MP PE) and co-occurring xenobiotics (tetracycline and [C₁₂TMA][Glyph]) on plant physiology, biochemical stress responses, and pollutant sorption behavior.The dataset consists exclusively of Excel (XLSX) files containing primary experimental measurements used for statistical analysis and data visualization. The files are organized according to analytical endpoints, as outlined below:Sorption experimentsHydroponic_sorption_Tetracycline.xlsx – concentrations of tetracycline in hydroponic medium over time (0 h, 1 h, 1 day, 7 days, 10 days) in systems with and without microplastics.Hydroponic_sorption_ILs.xlsx – concentrations of the ionic liquid [C₁₂TMA][Glyph] in hydroponic medium over time under analogous experimental conditions.Photosynthetic pigmentsHydroponic_chlorophyll.xlsx – chlorophyll a, chlorophyll b, and carotenoid contents in shoots of Brassica napus exposed to different treatments (control, microplastics, xenobiotics, humic acids, and their mixtures), used to calculate pigment ratios.Antioxidant enzymes and stress markersHydroponic_enzymes_CAT.xlsx – catalase (CAT) activity.Hydroponic_enzymes_APx.xlsx – ascorbate peroxidase (APx) activity.Hydroponic_enzymes_GST.xlsx – glutathione S-transferase (GST) activity.Hydroponic_enzymes_POx.xlsx – peroxidase (POx) activity.Hydroponic_enzymes_TBARS.xlsx – TBARS values representing lipid peroxidation and membrane damage.Secondary metabolites and osmotic stress indicatorsHydroponic_phenolic_compounds.xlsx – total phenolic content (TPC) in roots and shoots.Hydroponic_proline_content.xlsx – proline content as an indicator of osmotic and oxidative stress.Hydroponic_protein_content.xlsx – total soluble protein content in plant tissues.Toxicity screeningHydroponic_EC50.xlsx – EC₅₀ values derived from preliminary toxicity assays for tetracycline and [C₁₂TMA][Glyph], based on root and shoot length inhibition.