Characterization of the cellular responses produced by 17DD vaccination in C57Bl/6 mice.

C57Bl/6 mice were immunized on day 0 and boosted on day 21 with 104 PFUs of the human YF 17DD vaccine, and the splenocytes were tested in IFN-γ ELISPOT assays 7–10 days after the boost. The peptides used for the in vitro stimulation were 15-mers overlapping by 11 amino acids and comprising the entire length of the YF envelope protein. The total splenocyte, CD8-depleted splenocyte and CD4-depleted splenocyte populations were analyzed. The SFC values represents the average of two to four experiments performed with pools of three to five mice each.Characterization of the cellular responses produced by 17DD vaccination in C57Bl/6 mice.