Expanding Hematopoietic Stem Cell pool through Fine tuning of Tpo receptor with diabodies.
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
下载链接：
http://data.iscr.ac.cn/Article?id=4debb7d015107e2e5007ca21a8d70515
下载链接
链接失效反馈官方服务：
资源简介：
Class I cytokine receptors such as Thrombopoietin receptor (MPL) are dimers, but it is unclear whether remodeling receptor dimer topology is a viable strategy to "tune" signaling output. We utilized diabodies (DA) as surrogate ligands in a prototypical dimeric receptor-ligand system, the cytokine Thrombopoietin (TPO) and its receptor (TpoR), to dimerize TpoR ectodomains in non-native architectures. Diabody-induced signaling amplitudes varied from full to minimal agonism. Here we describe a single-cell transcriptome analysis of 659 human Hematopoietic Stem Cells cultured with different DA. We find several difference between differentiation and activation of signaling pathways compared to TPO.
提供机构：
Institute for Regenerative Medicine and Stem Cell Research, Stanford University School of Medicine
创建时间：
2022-02-20
