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Primers and PCR conditions used for the detection of target genes in porcine organ, white blood cell and digesta samples.

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NIAID Data Ecosystem2026-03-07 收录
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https://figshare.com/articles/dataset/_Primers_and_PCR_conditions_used_for_the_detection_of_target_genes_in_porcine_organ_white_blood_cell_and_digesta_samples_/312867
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1PCR conditions for rubisco F & R included 1 cycle at 94°C for 3 min, 35 cycles of 94°C for 30 s, down to 62°C for 30 s and back up to 72°C for 30 s and 1 cycle of 72°C for 7 min; cry1Ab-2 F & R; 1 cycle at 95°C for 3 min, 30 cycles of 95°C for 25 s, down to 62°C for 30 s and back up to 72°C for 45 s and 1 cycle of 72°C for 7 min.
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2015-12-02
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