S1 File - Lateralized cerebellar connectivity differentiates auditory pathways in echolocating and non-echolocating whales

S1 Text. Detailed cerebellar and subcortical projection sites in IC-cerebellar traces. S2 Text. Detailed cortical projections in IC-cerebellar traces. S3 Figures. Masked regions of interest in FSLeyes. S3 Figure A. D. delphis. Red= right, blue=left for cerebella, yellow=right and turquoise=left for inferior colliculi. S3 Figure B. S. Attenuata. Red= right, blue=left for cerebella, yellow=right and turquoise=left for inferior colliculi. S3 Figure C. L. acutus. Red= right, blue=left for cerebella, yellow=right and turquoise=left for inferior colliculi. S3 Figure D. B. borealis. Red= right, blue=left for cerebella, yellow=right and turquoise=left for inferior colliculi. S4 Figures. Ascending auditory tractograms. S4 Figure A1: D. delphis, left IC tracts shown in blue, right IC tracts shown in red, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S4 Figure A2: D. delphis, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S4 Figure A3: D. delphis, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S4 Figure A4: D. delphis, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S4 Figure B1: S. attenuata, left IC tracts shown in blue, right IC tracts shown in red, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S4 Figure B2: S. attenuata, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S4 Figure B3: S. attenuata, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S4 Figure B4: S. attenuata, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S4 Figure C1: L. acutus, left IC tracts shown in blue, right IC tracts shown in red, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S4 Figure C2: L. acutus, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S4 Figure C3: L. acutus, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S4 Figure C4: L. acutus, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S4 Figure D1: B. borealis, left IC tracts shown in blue, right IC tracts shown in red, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S4 Figure D2: B. borealis, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S4 Figure D3: B. borealis, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S4 Figure D4: B. borealis, left IC tracts shown in blue, right IC tracts shown in red, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S5 Figures. Contralateral collicular-cortical tractograms. S5 Figure A1: D. delphis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure A2: D. delphis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure A3: D. delphis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Still 3-dimensional view. S5 Figure A4: D. delphis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Rotating 3-dimensional view. S5 Figure B1: S. attenuata, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure B2: S. attenuata, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure B3: S. attenuata, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Still 3-dimensional view. S5 Figure B4: S. attenuata, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Rotating 3-dimensional view. S5 Figure C1: L. acutus, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure C2: L. acutus, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure C3: L. acutus, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Still 3-dimensional view. S5 Figure C4: L. acutus, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Rotating 3-dimensional view. S5 Figure D1: B. borealis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure D2: B. borealis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Orthographic view. S5 Figure D3: B. borealis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Still 3-dimensional view. S5 Figure D4: B. borealis, left IC to right cortex tracts shown in green, right IC to left cortex tracts shown in pink, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Both cerebella excluded. Rotating 3-dimensional view. S6 Figures. Contralateral collicular-cerebellar tractograms. S6 Figure A1. D. delphis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S6 Figure A2. D. delphis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S6 Figure A3. D. delphis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S6 Figure A4. D. delphis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S6 Figure B1. S. attenuata, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S6 Figure B2. S. attenuata, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S6 Figure B3. S. attenuata, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S6 Figure B4. S. attenuata, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S6 Figure C1. L. acutus, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S6 Figure C2. L. acutus, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S6 Figure C3. L. acutus, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S6 Figure C4. L. acutus, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S6 Figure D1. B. borealis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. Orthographic view. S6 Figure D2. B. borealis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Orthographic view. S6 Figure D3. B. borealis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Still 3-dimensional view. S6 Figure D4. B. borealis, left IC to right cerebellum tracts shown in orange, right IC to left cerebellum tracts shown in turquoise, set to a more liberal threshold of minimum 0.1% and maximum 5% of waytotals. Rotating 3-dimensional view. S7 Figures. Specific cerebellar targets in IC-cerebellar tractograms. S7 Figure A1. D. delphis, left IC to right cerebellum tracts shown in orange, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure A2. D. delphis, right IC to left cerebellum tracts shown in blue, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure B1. S. attenuata, left IC to right cerebellum tracts shown in orange, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure B2. S. attenuata, right IC to left cerebellum tracts shown in blue, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure C1. L. acutus, left IC to right cerebellum tracts shown in orange, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure C2. L. acutus, right IC to left cerebellum tracts shown in blue, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure D1. B. borealis, left IC to right cerebellum tracts shown in orange, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S7 Figure D2. B. borealis, right IC to left cerebellum tracts shown in turquoise, minimum threshold set to 1% and maximum threshold set to 30% of waytotals. S8 Tables. Results of the systematic tractographical analysis conducted for each specimen. S8 Table 1. Common dolphin. S8 Table 2. Pantropical spotted dolphin. S8 Table 3. Atlantic white-sided dolphin. S8 Table 4. Sei whale. S9 Table. Cerebellar projection sites and putative functions. S9 Table 1. Display of which cerebellar subregions were targeted in auditory-cerebellar traces, with columns further specifying the species involved, laterality in odontocetes versus mysticete, and putative function as informed by studies in other non-cetacean mammals [35, 83-84]. Asterisks in the “Laterality in Odontoceti” column indicate that the listed laterality was not consistently observed across all specimens, but rather represents the overall lateralization pattern. For example, the asterisk next to the “Left” designation in this column’s “Vermis” row reflects that the evidence for a left-vermis bias in odontocete auditory-cerebellar traces was somewhat mixed, given its left-side targeting in S. attenuata and its bilateral targeting in L. acutus.
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