Additional file 1 of Integrated characterization of filler tobacco leaves: HS–SPME–GC–MS, E-nose, and microbiome analysis across different origins

Additional file 1: Figure S1. FTLs sampling diagram, DHMS, DHRL, LCGM, PEJC, PENE, and YNYX respectively denote distinct geographical region names. Figure S2. Principal component analysis (PCA) based on chemical elements and chemical constituents of six regions of FTLs. Figure S3. The Linear Discriminant Analysis (LDA) plots of sensory evaluation of the six groups of FTLs. Figure S4. (A) The heatmap of volatile chemicals in the six groups fermented FTLs identified via HS–SPME–GC–MS detection. (B) Relative concentration of chemicals in the end of fermentation. (C) and (D) The Venn diagram of volatile chemicals in the six groups fermented FTLs. Figure S5. Venn plot of bacterial and fungal diversity at genera taxonomic level. Figure S6. The microbial NMDS2 (A, B) and NMDS3 (C, D) were linked to the geography longitude (A, C) and latitude (B, D). The lines represent the regression line fitted by the first-order polynomial. Table S1. Information of filler tobacco leaves (FTLs). Table S2. Analysis of free amino acids of the six groups FTLs. Table S3. Analysis of different species free amino acids in FTLs. Table S4. Analysis of chemical elements concentrations of the six groups FTLs. Table S5. Analysis of chemical constituents of the six groups FTLs. Table S6. Relative concentration of volatile chemicals in the six groups fermented FTLs identified via HS–SPME–GC–MS detection. Table S7. VIP values of volatile chemicals based on partial least squares-discrimination analysis model among six groups. Table S8. The numbers of microbes at phyla, class, family, genus and OTU level in six group fermented FTLs. Table S9. Alpha diversity indices of the microbial community in FTLs samples.