Microarray analysis of developing mouse cortex reveals regulation of ribosomal protein mRNAs throughout development

Neocortical development is spatiotemporally regulated by exogenous factors, but the mechanism regulating timed specificity of neocortical mRNA translation is unknown. We find that active mRNA translation sites (polysomes) contain ribosomal protein subsets that undergo dynamic spatiotemporal rearrangements during mouse neocortical development. Two samples of cortex total RNA each from four embryonic time points were collected.大脑新皮层发育受外源性因素的时空特异性调控，但目前调控新皮层信使RNA（mRNA）翻译时序特异性的分子机制仍未明确。本研究发现，活跃mRNA翻译位点（多聚核糖体，polysomes）内包含核糖体蛋白亚群，此类亚群在小鼠新皮层发育过程中会发生动态时空重排。我们收集了4个胚胎发育时间点的皮层总RNA样本，每个时间点各获取两份样本。