ChIP-seq analysis of dCas9 chromatin occupancy in HeLa cells expressing LAMPS

Significant enrichment of dCas9 chromatin occupancy at the targeted HS2 enhancer was observed in cells expressing LAMPS under the dark condition and after blue light illumination. ChIP-seq was performed to determine the chromatin occupancy of biotinylated dCas9 in cells expressing target-specific sgRNAs or no sgRNA control.