MyoD ChIP-chip on Ciona intestinalis embryo (early-mid gastrula). Ciona intestinalis

A genomic overview of in vivo binding of a transcription factor MyoD in the ascidian gastrula embryo. MyoD is known to be essential and sufficient for differentiation of the muscle. Keywords: ChIP-chip Overall design: Fertilized eggs were electroporated with a construct encoding GFP-tagged MyoD. After fixation, Chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE DNA were amplified by LM-PCR for the Chip analysis. Two independent experiments were performed to reduce experimental errors.