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资源简介：
Minimize off-target edits for base editors
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创建时间：
2023-03-10
相关数据集
Selective recruitment of the AAA+ ATPase TnsC increases the fidelity of Type I-F CRISPR RNA-guided transposition
Bacterial transposons are pervasive mobile genetic elements that exploit distinct DNA binding proteins for their horizontal spread. For example, E. coli Tn7 homes to a specific attachment site using a
NIAID Data Ecosystem110
Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR/Cas9 genome editing. Homo sapiens
Gene disruption by CRISPR/Cas9 is highly efficient and relies on the error-prone non-homologous end-joining (NHEJ) pathway. Conversely, precise gene editing requires homology-directed repair (HDR), wh
NIAID Data Ecosystem100
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
Even the latest generation of base editor (BE3) causes unwanted indels and non-C-to-T substitutions, compromising the fidelity of base editing outcome. Here we report a enhanced base editing system. T
NIAID Data Ecosystem60
High-fidelity base editing mediated by Cpf1-cytidine deaminase fusion
C-to-T base editing mediated by CRISPR/Cas9 base editors (BEs) needs a G/C-rich PAM and the editing fidelity is compromised by unwanted indels and non-C-to-T substitutions. We developed CRISPR/Cpf1-ba
NIAID Data Ecosystem110
Secondary Conformational Checkpoint in CRISPR-Cas9
A specific checkpoint between target DNA binding and cleavage primarily governs the precision of Cas9 gene editing. Although various CRISPR-Cas9 variants have been developed to improve DNA cleavage ac
Figshare2024-04-16 更新70
