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资源简介：
Minimize off-target edits for base editors
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创建时间：
2023-03-10
相关数据集
Engineering a miniaturized SaCas9 adenine base editor with reduced RNA off-targets and increased on-target DNA editing efficiencies. Protein domain-altered adenine base editors
We compared the editing profile of circularly permuted and domain inlaid Cas9 base editors, and found that on-target editing can be largely maintained following their intradomain insertion, but that s
NIAID Data Ecosystem80
Selective recruitment of the AAA+ ATPase TnsC increases the fidelity of Type I-F CRISPR RNA-guided transposition
Bacterial transposons are pervasive mobile genetic elements that exploit distinct DNA binding proteins for their horizontal spread. For example, E. coli Tn7 homes to a specific attachment site using a
NIAID Data Ecosystem60
Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [cas9_variants]
Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing
NIAID Data Ecosystem40
Engineering precise adenine base editors with infinitesimal bystander mutation and off-target editing
Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications with the development of highly efficient and compatible adenine deaminase TadA-8e. However, they also induce bystande
NIAID Data Ecosystem70
On targeting specificity of Cas12b nucleases
On targeting specificity of Cas12b nucleases
NIAID Data Ecosystem50
