Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (ATAC-Seq)

In this experiment, the effect of reduced CDX2 expression on chromatin accessibility in trophoblast stem cells (TSCs) was assayed using ATAC-seq. Overall design: High read depth transposase accessible chromatin profiles were generated using 50,000 cells. shRNA vectors containing a constitutively active mCherry cassette to visualise and isolate transfected and non-transfected populations. Positively transfected CDX2 knockdown TSCs were compared with control transfected and untransfected TSC populations.