Transcriptomics analysis of aged asthmatic lung and treated with dexamethasone during HDM induced airway inflammaton

This study was designed to evaluate the lung transcriptome analysis against dexamethasone treatment throughout HDM challenge period in the aged (80-82 Weeks) mice. The lung samples from aged inflammed mice with or without dexamethasone treatmrnt were isolated followed by RNA isolation and RNA sequence analysis. Overall design: First, we sensitized aged (80-82 Weeks) mice with 50 µg of HDM in a volume of 40 µL through intranasal (i. n) route on days 1, 3, and 5. The challenge phase consisted of 3 consecutive phases of 3 doses (i.n) of HDM (50 µg) on days 11-13, 18-20, and 25-27 respectively. Dexamethasone (4 mg/Kg body weight, intraperitoneal, route) treatment was given throughout the challenge period after every 72 h interval. Post 24 h, last challenge all the lung samples were collected for futher processing. Further, RNA was isolated, checked for RNA quality and processed for RNA sequence analysis.