Supplement of manuscript 521330

This folder contains analysis data from the study submitted as manuscript (Manuscript ID 521330):

TITLE:

Comparative metabarcoding and metatranscriptomic analysis of microeukaryotes within coastal surface waters of West Greenland and Northwest Iceland

AUTHORS:

Stephanie Elferink, [e-mail], Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research  

Sylke Wohlrab, [e-mail], Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

Stefan Neuhaus, [e-mail], Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research  

Allan Cembella, [e-mail], Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research  

Lars Harms, [e-mail], Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

Uwe John, [e-mail], Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, and Helmholtz Institute for Functional Marine Biodiversity

JOURNAL:

Frontiers in Marine Science, section Marine Molecular Biology and Ecology

MS-ID:

521330

HOWTO:

Sequences identified as Alveloates or Stramenopiles (description in manuscript) had been were classified more accurately by PhyloAssigner version 6.166 (https://github.com/jungbluth/phyloassigner, Vergin et al., 2013, DOI:10.1038/ismej.2013.32) with a phylogenetic placement onto reference trees based on 18S/28S concatenated alignments, according to Elferink et al. 2017 (DOI: 10.1016/j.dsr.2016.11.002).

CONTENT:

reference databases:

- Alveolata_SSU-LSU-concat_310715_636.phyloassignerdb

- Stramenopiles_SSU_LSU_concat_030815_1777.phyloassignerdb

query sequence files:

- Alveolata_seqtab_SIGN_dada2.fasta

- Alveolata_seqtab_SIGN_dada2.fasta

created out put folder including the taxonomic annotation:

- Alveolata_seqtab_SIGN_dada2.place.out

- Stramenopiles_seqtab_SIGN_dada2.place.out

text file containing the used commands:

- commands