Streamlined low-input transcriptomics through EASY-RNAseq

we describe a streamlined RNAseq protocol (EASY RNAseq) for sensitive transcriptome assessment starting from low amount of input materials. EASY RNAseq is technically robust enough for sequencing small pools of homogenous and heterogeneous cells, recovering higher numbers of genes and with a more even expression distribution pattern than other commonly used methods. To evaluate the capbility of EASY-RNASeq respect to the amount of input materials, different amount of input materials extracted from mice spleen(0.1ng, 0.5ng, 1.0ng, 5.0ng) were used for constructing EASY-RNASeq libraries. And for cells sample, different number of input cells obtained from murine CD3+ T cells(1e2, 5e2, 1e3, 1e4) were used to do EASY-RNASeq library construction. All libraries were sequenced by Illumina platform.