Genome-wide H3K9me2 mapping in single deletions of the selected chromatin modulating genes, histone H3 mutants and ccp1Δepe1Δ double mutant

We report the high-throughput profiling of H3K9me2 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 maps in single deletions of the selected chromatin modulating genes, histone H3 mutants and ccp1Δepe1Δ double mutant in fission yeast. We find that the subtelomeric heterochromatin distribution is highly variable under different genetic perturbations and small heterochromatin islands are formed at clr4 and clr2 loci in ccp1Δepe1Δ. Single deletions of the selected chromatin modulating genes, histone H3 mutants and ccp1Δepe1Δ double mutant were examined by anti-H3K9me2 ChIP-seq in fission yeast.