Masayuki J. Sato, Hidekazu Kuwayama, Wouter N. van Egmond, Airi L. K. Takayama, Hiroaki Takagi, Peter J. M. van Haastert, Toshio Yanagida, Masahiro Ueda (2011) CIL:24046, Dictyostelium discoideum AX2, amoeboid cell. CIL. Dataset

Starved Dictyostelium cells lacking both guanylyl cyclases (GCase A and GCase), but expressing GCase with an inactivated catalytic domain (sGCΔCat) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate continuously toward the anode (right). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) In contrast, cathode-directed migration is strongly attenuated in wild-type cells in the same medium. Compare with wild-type control video (CIL# 24043) and other mutants lacking guanylyl cyclase activity (CIL#24044, 24045). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.

在缺乏两种鸟苷酸环化酶（GCase A 和 GCase）的饥饿型 Dictyostelium 细胞中，当这些细胞表达具有失活催化域的 GCase（sGCΔCat）并处于补充有 60 µM LY294002（一种 PI3K 抑制剂）和 1µM cAMP 的培养基中时，会持续地向阳极（右侧）迁移。施加了 10 Vpcm 的直流电场，并在 5 秒间隔内对细胞进行了 20 分钟的观察。（比例尺，50 µm。）相比之下，野生型细胞在同一培养基中向阴极迁移的现象显著减弱。可与野生型对照组视频（CIL# 24043）以及缺乏鸟苷酸环化酶活性的其他突变体（CIL#24044, 24045）进行比较。视频作为《美国国家科学院院刊》（Proc Natl Acad Sci，2009）第 106 卷第 6667-6672 页的辅助信息。