T-cell receptors targeting distinct FLT3-D835Y neoantigens display differential reactivity toward acute myeloid leukemia_flow_cytometry_data_1

Figure 2A: FACS staining of FLT3-D835Y-specific T-cell clones (YIMSDSNYV, HLA-A*02:01). Fluorochromes: PE = tetramer, Pacific Blue = CD8, Live-Dead BLUE = viability dye. Figure 2B: FACS staining of FLT3-D835Y-specific T-cell clones (FGLARYIM, HLA-B*08:01). Fluorochromes: PE = tetramer, Pacific Blue = CD8, Pacific Orange = viability dye. Figure 4A / Figure S6: FACS staining of TCR-T cells transduced with FLT3-D835Y-specific TCRs. Fluorochromes: PE = tetramer, Pacific Blue = CD8, Zombie Red = viability dye, APC = mTCRcbeta, PerCP-Cy5.5 = humanTCRalphabeta. Figure 5E / Figure S9A: FACS staining of MOLM13-RES-AC lysis assay with FLT3-A2 TCR-T cells. Fluorochromes: Zombie Red = viability dye, Pacific Blue = CD8, PE = CD33. Figure 5F / Figure S9B: FACS staining of MOLM13-RES-AC lysis assay with FLT3-B8 TCR-T cells. Fluorochromes: Zombie Red = viability dye, Pacific Blue = CD8, PE = CD33.