R1 Supplemental dataset.xlsx
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https://figshare.com/articles/dataset/R1_Supplemental_dataset_xlsx/7464818
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资源简介：
Whole transcriptome analysis of TGF-beta1 treated fibroblasts
Methods: RNA was purified using a miRCURY RNA
isolation kit (#300110, Exiqon A/S, Vedbaek, Denmark) with an Agilent 2100
Bioanalyzer (Agilant, Santa Clara, CA, USA) used to check RNA integrity. The
Illumina TrueSeq mRNA sample preparation kit (Illumina, San Diego, CA, USA) was
used to prepare RNA libraries for sequencing on the Illumina HiSEq 2000. Reads
were aligned using TopHat2 with the rn6 build of the rat genome. Transcript
assembly and differential expression (DE) was by Cufflinks with Refseq mRNAs to
guide assembly. Statistically different changes were identified by ANOVA using
Partek Flow (Partek, Singapore), as previously described in https://doi.org/10.1016/j.biocel.2017.09.009.
创建时间：
2019-02-24
